Title : Exploring the therapeutic potential of FBBR in mitigating fine dust-induced inflammation in SH-SY5Y cells and enhancing cognitive function following intranasal instillation of pm2.5 in mice
Abstract:
The escalating prevalence of particulate matter (PM) has prompted significant apprehensions regarding its detrimental impact on human health. This research delves into the potential of FBBR in alleviating the effects induced by PM2.5 in both SH-SY5Y cells and mice exposed to PM2.5. A battery of assays, encompassing MTT, NO, western blot, ELISA, and behavioral studies (MWM and Y-maze), was executed. Results revealed that PM2.5 induced pronounced cytotoxicity and heightened nitric oxide (NO) production at a concentration of 100 μg/mL in SH-SY5Y cells. Moreover, FBBR administration effectively mitigated PM2.5-induced cytotoxicity and suppressed NO production in SH-SY5Y cells. In a mice model exposed to intranasal instillation of 10 mg/kg body weight of PM2.5, cognitive impairments ensued. Nevertheless, FBBR treatment ameliorated these impairments in both the Y-maze and MWM tests in PM2.5-exposed mice. Additionally, FBBR administration upregulated the expression of brain-derived neurotrophic factor (BDNF) and downregulated inflammatory markers in the brains of PM2.5-exposed SH-SY5Y cells. These findings underscore the detrimental impact of PM2.5 on the nervous system and propose FBBR as a potential nutraceutical agent for mitigating these effects. Crucially, the results accentuate the imperative need to address the harmful consequences of PM2.5 on the nervous system and underscore the promising role of FBBR as a protective intervention against the adverse effects associated with PM2.5 exposure.
