Title : Deciphering neuroinflammation: Investigating the impact of humoral immune factors on the development and therapy of experimental autoimmune encephalomyelitis, with a focus on the ganglioside-containing drug cronassial
Abstract:
Special attention is directed towards understanding the mechanisms underlying the development of multiple sclerosis, with a particular focus on humoral immune factors, including cytokines and circulating immune complexes (CICs). These factors play a pivotal role in the processes of neuroinflammation and neurodegeneration during both acute and chronic stages of the disease. Substantial alterations in the profile of proinflammatory cytokines and CICs are evident throughout the course of pathology. The objective of this study was to explore the concentrations of proinflammatory cytokines (IL-1β, IL-6, TNFα) in blood plasma and homogenates of the brain and spinal cord, as well as CICs in serum. Additionally, histological analysis with Bcl2 staining was performed in experimental autoimmune encephalomyelitis and its treatment. The therapeutic agent employed was Cronassial, which contains mono-di-tri-sialyl gangliosides. The data obtained indicate the neuroprotective effects of Cronassial when administered to animals with experimental autoimmune encephalomyelitis.
Audience Take Away Notes:
- Increase in Concentrations of Humoral Immune Factors: Our study demonstrated that the pathology is accompanied by an increase in the concentrations of the studied humoral immune factors in the plasma and blood serum, as well as in the homogenates of the brain and spinal cord of experimental animals.
- Impact on Inflammatory Processes and Demyelinating Plaques: This amplifies inflammatory infiltration and contributes to the formation and enlargement of demyelinating plaques in tissues, leading to excitotoxicity and neuroinflammation at the pathological level.
- Histochemical Analysis and Bcl-2 Positive Staining: Histochemical analysis revealed Bcl-2 positive staining in the cells of spinal and brain tissues.
- Neuroprotective Effect of Drug Administration: Administration of a drug containing ganglioside in vivo leads to the normalization of the levels of the studied factors, demonstrating a neuroprotective effect.
