Title : Ameliorative potential of kalonji seed oil against bifenthrin-induced pituitary histopathologies in albino mice
Abstract:
Histopathological and micrometric alterations of mice pituitary gland against two dose groups of bifenthrin 2.5mg/kg and 5mg/kg (Bf2.5 and Bf5) exposure were investigated. Additionally, the rescuing potential of kalonji seed oil (k) was also investigated. The study group comprised 60 male albino mice (27–30g) randomly distributed in 6 groups (n=10). (i) Vc (vehicle control) group was maintained and treated with 0.1mL of corn oil only for 1–14 days; (ii) K (positive control) group was treated with0.1mL of corn oil for 1–7 days and 0.1ml of 10% kalonji seed oil mixed in corn oil for next8–14 days; (iii) Bf2.5 group and (iv) Bf5 groups were treated with 2.5mg/kg and 5mg/kg Bf solutions in corn oil of the respective doses of insecticide for 1–7 days followed by0.1mL of corn oil on 8–14 days; (v) BfK2.5 an d (vi) BfK5 groups animals were treated with 0.1mL of respective doses of Bf on 1–7 days followed by 10% kalonji seed oil solution as in the positive control group. All animals were sacrificed on day 15 to recover the pituitary gland. Results show various histopathological alterations in pituitary gland related to Bf exposure. The characteristic pathologies of pituitary discovered in this study include cellular vacuolations causing hypertrophy finally leading to secondary atrophy and adeno-hypophyseal tissue damage in terms of focal degenerations in the typically arranged nest and cords of the endocrine cells. The cell nuclei were slightly elliptical instead of typical rounded appearance having less densely stained sinusoid. Analysis of the data showed a significant decline (p ≤0.05) in total number of pituicytes per unit area (2500μm2) and relative area occupied by pituicytes inBf2.5 (15.26± 0.63 and 964±72, respectively), Bf5 (14.4±0.51 and 852±54, respectively), as compared to the vehicle Vc (20.6±0. 73 and 1375±108, respectively) and K group (21.46±0.72 and 1454±74, respectively). A substantial recovery in the total number of pituicytes per unit area (2500μm2) and relative area occupied by pituicytes was present in the BfK2.5 group (18.9±0.57 and 1234±67, respectively) and BfK5 group (16.2±0.61 and852±54, respectively). A similar pattern of significant (p≤0.05) alterations was present in the analysis of the micrometric data for number of acidophils, basophils and chromophobes in pituitary gland. On the basis of these findings, it is concluded that insecticide harbor pituitary gland histopathological and micrometric potentials that are at least partially ameliorated by kalonji oil treatment. These findings thus indicate effective neutraceutical potential of kalonji oil for possible human consumption in similar accidental exposure to the insecticide.
